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Haiwei Gu, PhD

Assistant Professor, School of Nutrition and Health Promotion

Info

 (480) 301-6016

Education

  • PhD Chemical Physics/Bioanalytical Chemistry, Purdue University 2008
  • BS Applied Physics, University of Science and Technology of China 2003

Biography

My research interests focus on MS-based metabolomics and its applications in early disease diagnosis, drug metabolism, and biological sciences. I am working closely with a number of biological researchers and clinical practitioners in various studies for identifying metabolic markers, which are useful for detecting cancers, investigating cancer mechanisms using cell/mitochondria models, examining metabolic remodeling during pressure-overload hypertrophy, comprehensively profiling age-dependent changes of cardiac metabolites, etc.

Research Interests

The Gu research group focuses on mass spectrometry (MS)-based metabolomics and its applications for biomarker discovery and systems biology research. We are skilled in the development, optimization, and applications of MS methods for both qualitative and quantitative measurements. We utilize a range of platforms, including LC- and GC-MS for global aqueous profiling and lipidomics. We have developed a number of targeted LC-MS/MS assays to detect panels of metabolites, including a 200+ aqueous metabolite assay that interrogates 35 metabolic pathways, as well as assays to measure bile acids, acyl carnitines, co-enzymes, and cardiolipins. Recent methods developed by our group include quantitative methods to measure metabolite concentrations, metabolic flux analysis approaches, and ratio analysis methods for unknown identification. In addition, we have extensive experience with advanced multivariate statistical analysis methods, including PCA, PLS-DA, SVM, etc. We can proficiently program in Matlab, R, and Pathyn to analyze MS and NMR data, both individually and in combination.

In addition to our methods development activities, we are working closely with a number of biological researchers and clinical practitioners in various studies. We have been active in the identification of biomarkers for early cancer detection, recurrence monitoring, and therapy prediction. We are also involved in studies of heart diseases, obesity, and diabetes, as well as nutrition based disease prevention and aging. Our group has a strong interest in expanding the understanding of disease risk factors using metabolite profiling approaches. My group has experience running large metabolomics sample sets that incorporate a number of quality control measures to ensure reliability of large datasets. Our work in metabolomics is thus applied to a broad range of applications in both basic and clinical metabolic research.

Research Publications

  1. J. R. Chao*, K. Knight, A. L. Engel, C. Jankowski, Y. Wang, M. A. Manson, H. Gu, D. Djukovic, D. Raftery, J. B. Hurley, J. Du*, “Human retinal pigment epithelial cells prefer proline as a nutrient and transport metabolic intermediates to the retinal side”, J. Bio. Chem., 292, 12895-12905, 2017. PMCID: PMC5546030
  2. F. Miles, S. Navarro, Y. Schwarz, H. Gu, D. Djukovic, T. Randolph, A. Shojaie, M. Kratz, M. Hullar, P. Lampe, M. Neuhouser, D. Raftery, J. Lampe*, “Plasma metabolite abundances are associated with urinary enterolactone excretion in healthy participants on controlled diets”, Food Funct., 8, 3209-3218, 2017. PMID: 28808723
  3. M. Buas, H. Gu, D. Djukovic, J. Zhu, L. Onstad, B. Reid, D. Raftery*, T. Vaughan*, “Candidate serum metabolite biomarkers for differentiating gastroesophageal reflux disease, Barrett's esophagus, and high-grade dysplasia/esophageal adenocarcinoma”, Metabolomics, 13, 23, 2017. PMCID: PMC5295138
  4. H. Lu, H. Zhu*, T. Zhu, Y. Xiao, S. Xie, H. Gu, M. Cui, L. Luo, “Metabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry”, Sci. Reports, 7, 5136, 2017. PMCID: PMC5506000.
  5. T. Li, Z. Zhang, S. Kolwicz, L. Abell, N. Roe, M. Kim, B. Zhou, Y. Cao, J. Ritterhoff, H. Gu, D. Raftery, H. Sun, R. Tian*, “Defective Branched-Chain Amino Acid Catabolism Disrupts Glucose Metabolism and Sensitizes the Heart to Ischemia-Reperfusion Injury”, Cell Metab., 25, 374-385, 2017. PMCID: PMC5301464.
  6. J. Rabinowitz, A. Robitaille, Y. Wang, C. Ray, R. Thummel, H. Gu, D. Djukovic, D. Raftery, J. Berndt, R. Moon*, “Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish”, PNAS, 114, E717-E726, 2017. PMCID: PMC5293114.
  7. H. Zhao, J. Shen, D. Djukovic, C. Daniel-MacDougall, H. Gu, X. Wu, W. H. Chow, “Metabolomics-identified metabolites associated with body mass index and prospective weight gain among Mexican American women”, Obes. Sci. & Pract., 2, 309-317, 2016. PMCID: PMC5043515
  8. H. Gu, J. Du, P. A. Carroll, J. Zhu, F. Carnevale Neto, R. N. Eisenman, D. Raftery*, “Quantitative Method to Investigate the Balance between Metabolism and Proteome Biomass: Starting from Glycine”, Angew. Chem. Int. Ed., 55, 15656-15650, 2016. PMID: 27860107.
  9. L. Deng+, H. Gu+*, J. Zhu, G. A. Nagana Gowda, D. Djukovic, E. G. Chiorean, D. Raftery*, “Combining NMR and LC/MS Using Backward Variable Elimination: Metabolomics Analysis of Colorectal Cancer, Polyps, and Healthy Controls”, Anal. Chem., 88, 7975-7983, 2016. PMID: 27437783
  10. M. Buas, H. Gu, D. Djukovic, J. Zhu, C. W. Drescher, N. Urban, D. Raftery, C. Li*, “Identification of Novel Candidate Plasma Metabolite Biomarkers for Distinguishing Serous Ovarian Carcinoma and Benign Serous Ovarian Tumors”, Gynecol. Oncol., 140, 138-144, 2016. PMID: 26521694
  11. M. Heitkemper*, C. Han, M. Jarrett, H. Gu, D. Djukovic, R. Shulman, D. Raftery, W. Henderson, K. Cain, “Serum Tryptophan Metabolite Levels During Sleep in Patients With and Without Irritable Bowel Syndrome (IBS)”, Biol. Res. Nurs., 18, 193-198, 2016. PMID: 26156003
  12. J. Du, A. Rountree, W. M. Cleghorn, L. Contreras, K. J. Lindsay, M. Sadilek, H. Gu, D. Djukovic, D. Raftery, J. Satrústegui, M. Kanow, L. Chan, S. H. Tsang, I. R. Sweet, J. B. Hurley*, “Phototransduction Influences Metabolic Flux and Nucleotide Metabolism in Mouse Retina”, J. Bio. Chem., 291, 4698-4710, 2016. PMCID: PMC4813492
  13. Y. Chiao, S. C. Kolwicz, N. Basisty, A. Gagnidze, J. Zhang, H. Gu, D. Djukovic, R. P. Beyer, D. Raftery, M. MacCoss, R. Tian, P. S. Rabinovitch*, “Rapamycin Transiently Induces Mitochondrial Remodeling to Reprogram Energy Metabolism in Old Hearts”, Aging, 8, 314-327, 2016. PMID: 26872208
  14. S. Lim, C. Li, W. Xia, H. Lee, S. Chang, J. Shen, J. Hsu, D. Raftery, D. Djukovic, H. Gu, W. Chang, H. Chang, H. Wang, M. Chen, L. Huo, C. Chen, Y. Wu, A. Sahin, S. Hanash, G. Hortobagyi, M. Hung*, “EGFR Signaling Enhances Aerobic Glycolysis in Triple-Negative Breast Cancer Cells to Promote Tumor Growth and Immune Escape”, Cancer Res., 76, 1284-1296, 2016. PMCID: PMC4775355
  15. H. Gu*, P. Zhang, J. Zhu, D. Raftery*, “Globally Optimized Targeted Mass Spectrometry (GOT-MS): Reliable Metabolomics Analysis with Broad Coverage”, Anal. Chem., 87, 12355-12362, 2015. PMID: 26579731
  16. H. Sperber, J. Mathieu, Y. Wang, A. Ferreccio, J. Hesson, Z. Xu, K. A. Fischer, A. Devi, D. Detraux, H. Gu, S. L. Battle, M. Showalter, C. Valensisi, J. H. Bielas, N. G. Ericson, L. Margaretha, A. M. Robitaille, D. Margineantu, O. Fiehn, D. Hockenbery, C. A. Blau, D. Raftery, A. A. Margolin, R. D. Hawkins, R. T. Moon, C. B. Ware, H. Ruohola-Baker*, “The Metabolome Regulates the Epigenetic Landscape During Naive-to-Primed Human Embryonic Stem Cell Transition”, Nat. Cell Bio., 17, 1523-1535, 2015. PMCID: PMC4662931
  17. J. Zhu, D. Djukovic, L. Deng, H. Gu, F. Himmati, M. Abu Zaid, E. G. Chiorean, D. Raftery*, “Targeted Serum Metabolite Profiling and Sequential Metabolite Ratio analysis for Colorectal Cancer Progression Monitoring”, Anal. Bioanal. Chem., 407, 7857-7863, 2015. PMID: 26342311
  18. J. Wang, Q. Li, H. Gu, X. Guo, S. Yang*, Z. Wang, “Direct Analysis of Micro Area on Tooth Surface by Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry”, Chem. J. Chin. Univ., 36, 1067-1073, 2015.
  19. H. Gu*, J. Du, F. Carnevale Neto, P. Carroll, S. Turner, G. Chiorean, R. Eisenman, D. Raftery, “Metabolomics Method to Comprehensively Analyze Amino Acids in Different Domains”, Analyst, 140, 2726-2734, 2015. PMCID: PMC4380628
  20. P. Carroll, D. Diolaiti, L. McFerrin, H. Gu, D. Djukovic, J. Du, P. Cheng, S. Anderson, M. Ulrich, J. Hurley, D. Raftery, D. Ayer, R. Eisenman*, “Deregulated Myc Requires MondoA/Mlx for Metabolic Reprogramming and Tumorigenesis”, Cancer Cell, 27, 271-285, 2015. PMCID: PMC4326605
  21. N. Reyes, G. Banks, M. Tsang, D. Margineantu, H. Gu, D. Djukovic, J. Chan, M. Torres, H. Liggitt, D. Hirenallur-S, D. Hockenbery, D. Raftery, B. Iritani*, “Fnip1 Regulates Skeletal Muscle Fiber Type Specification, Fatigue Resistance, and Susceptibility to Muscular Dystrophy”, , 112, 424-429, 2015. PMCID: PMC4299192
  22. R. Sood, H. Gu, D. Djukovic, L. Deng, M. Ga, L. Muffley, D. Raftery, A. Hocking*, “Targeted Metabolic Profiling of Wounds in Diabetic and Nondiabetic Mice”, Wound Repair Regen., 23, 423-34, 2015. PMID: 25845676
  23. S. Barton, S. Navarro, M. Buas, Y. Schwarz, H. Gu, D. Djukovic, D. Raftery, M. Kratz, M. Neuhouser, J. Lampe*, “Targeted Plasma Metabolome Response to Variations in Dietary Glycemic Load in a Randomized, Controlled, Crossover Feeding Trial in Healthy Adults”, Food Funct., 6, 2949-2956, 2015. PMID: 26165375
  24. S. Pan, Y. Tian, M. Li, J. Zhao, L. Zhu, W. Zhang, H. Gu*, H. Wang, J. Shi, X. Fang, P. Li, H. Chen, “Quantitative Detection of Nitric Oxide in Exhaled Human Breath by Extractive Electrospray Ionization Mass Spectrometry”, Sci. Reports, 5, 8725, 2015. PMCID: PMC4348632
  25. C. Chen, L. Deng, S. Wei, G.A.N. Gowda, H. Gu, E.G. Chiorean, M. Abu Zaid, M. Harrison, J. Pekny, P. Patrick, D. Zhang, M. Zhang*, D. Raftery*, “Exploring Metabolic Profile Differences between Colorectal Polyp Patients and Controls Using Seemingly Unrelated Regression”, J. Proteome Res., 14, 2492-2499, 2015. PMID: 25919433
  26. W. Yao, H. Gu, J. Zhu, G. Barding, H. Cheng, B. Bao, L. Zhang*, A. Ding*, W. Li, “Integrated Plasma and Urine Metabolomics Coupled with HPLC/QTOF-MS and Chemometric Analysis on Potential Biomarkers in Liver Injury and Hepatoprotective Effects of Er-Zhi-Wan”, Anal. Bioanal. Chem., 406, 7367-7378, 2014. PMID: 25245419
  27. J. Zhu, D. Djukovic, L. Deng, H. Gu, F. Himmati, E. Chiorean, D. Raftery*, “Colorectal Cancer Detection Using Targeted Serum Metabolic Profiling”, J. Proteome Res., 13, 4120-4130, 2014. PMID: 25126899
  28. D. Dai, P. Karunadharma, Y. Chiao, N. Basisty, D. Crispin, E. Hsieh, T. Chen, H. Gu, D. Djukovic, D. Raftery, R. Beyer, M. Maccoss, P. Rabinovitch*, “Altered Proteome Turnover and Remodeling by Short-term Caloric Restriction or Rapamycin Rejuvenate the Aging Heart”, Aging Cell, 13, 529-539, 2014. PMCID: PMC4040127
  29. H. Gu, A. N. Gowda, F. Carnevale Neto, M. Opp, D. Raftery*, “RAMSY: Ratio Analysis of Mass Spectrometry to Improve Compound Identification”, Anal. Chem., 85, 10771-10779, 2013. PMCID: PMC3867450
  30. N. Xu, Z. Zhu, S. Yang, J. Wang, H. Gu*, H. Chen, “Direct Detection of Amino Acids Using Extractive Electrospray Ionization Tandem Mass Spectrometry”, Chin. J. Anal. Chem., 41, 523–528, 2013.
  31. H. Baniasadi, A. N. Gowda, H. Gu, A. Zeng, S. Zhuang, N. Skill, M. Maluccio, D. Raftery*, “Targeted Metabolic Profiling of Hepatocellular Carcinoma and Hepatitis C using LC-MS/MS”, Electrophoresis, 34, 2910-2917, 2013. PMCID: PMC3826436
  32. H. Zhang, H. Gu, F. Yan, N. Wang, Y. Wei, J. Xu, H. Chen*, “Direct Characterization of Bulk Samples by Internal Extractive Electrospray Ionization Mass Spectrometry”, Sci. Reports, 3, 2495, 2013. PMCID: PMC3750536
  33. M. Li, J. Ding, H. Gu, Y. Zhang, S. Pan, N. Xu, H. Chen*, H. Li, “Facilitated Diffusion of Acetonitrile Revealed by Quantitative Breath Analysis Using Extractive Electrospray Ionization Mass Spectrometry”, Sci. Reports, 3, 1205, 2013. PMCID: PMC3564039
  34. Y. Qi, H. Gu, Y. Song, X. Dong, A. Liu, Z. Lou, G. Fan, Y. Chai*, “Metabolomics Study of Resina Draconis on Myocardial Ischemia Rats Using Ultraperformance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry Combined with Pattern Recognition Methods and Metabolic Pathway Analysis”, Evid. Based Complement. Alternat. Med., 438680, 2013. PMCID: PMC3677627
  35. F. Tayyari, A. N. Gowda, H. Gu, D. Raftery*, “15N-Cholamine-A Smart Isotope Tag for Combining NMR- and MS-Based Metabolite Profiling”, Anal. Chem., 85, 8715-8721, 2013. PMCID: PMC3803152
  36. H. Gu, A. N. Gowda, D. Raftery*, “Metabolic Profiling: Are We en route to Better Diagnostic Tests for Cancer?”, Future Oncol., 8, 1207-1210, 2012. PMID: 23130920
  37. H. Gu, N. Xu, H. Chen*, “Direct Analysis of Biological Samples Using Extractive Electrospray Ionization Mass Spectrometry (EESI-MS)”, Anal. Bioanal. Chem., 403, 2145-2153, 2012. PMID: 22434271
  38. H. Gu, Qi, Y. Qi, N. Xu, J. Ding, Y. An, H. Chen*, “Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry-Based Metabolomics for Cancer Diagnosis”, Chin. J. Anal. Chem., 40, 1933–1937, 2012.
  39. C. Liu, X. Zhang, S. Xiao, B. Jia, S. Cui, J. Shi, N. Xu, X. Xie, H. Gu*, H. Chen, “Detection of Trace Levels of Lead in Aqueous Liquids Using Extractive Electrospray Ionization Tandem Mass Spectrometry”, Talanta, 98, 79-85, 2012. PMID: 22939131
  40. Z. Zhu, J. Han, Y. Zhan, Y. Zhou, N. Xu, B. Zhang, H. Gu, H. Chen*, “Sensitive Ionization of Non-volatile Analytes Using Protein Solutions as Spray Liquid in Desorption Electrospray Ionization Mass Spectrometry”, Rapid Commun. Mass Spectrom., 26, 2770-2776, 2012. PMID: 23124668
  41. H. Gu, Y. Huang, M. Filgueira, P. W. Carr*, “Effect of First Dimension Phase Selectivity in Online Comprehensive Two Dimensional Liquid Chromatography (LCxLC)”, J. Chrom. A., 1218, 6675-6687, 2011. PMCID: PMC3256932
  42. H. Gu, Z. Pan, B. Xi, V. Asiago, B. Musselman, D. Raftery*, “Principal Component Directed Partial Least Squares Analysis for Combining NMR and MS Data in Metabolomics: Application to the Detection of Breast Cancer”, Anal. Chim. Acta, 686, 57-63, 2011. PMCID: PMC3040422
  43. Y. Huang, H. Gu, M. Filgueira, P. W. Carr*, “An Experimental Study of Sampling Time Effects on the Resolving Power of Online Two-Dimensional HPLC”, J. Chrom. A., 1218, 2984-2994, 2011. PMCID: PMC3132878
  44. B. Hu, S. Yang, M. Li, H. Gu, H. Chen*, “Direct Detection of Native Proteins in Biological Matrices Using Extractive Electrospray Ionization Mass Spectrometry”, Analyst, 136, 3599-3601, 2011. PMID: 21734987
  45. H. Gu, Y. Huang, P. W. Carr*, “Peak Capacity Optimization in Comprehensive Two Dimensional Liquid Chromatography: A Practical Approach”, J. Chrom. A., 1218, 64-73, 2010. PMCID: PMC3034304
  46. H. Gu, S. Yang, J. Li, B. Hu, H. Chen*, L. Zhang, Q. Fei, “Geometry Independent Neutral Desorption Device Sampling Explosives on Various Surfaces for Sensitive EESI-MS Detection”, Analyst, 135, 779-788, 2010. PMID: 20349542
  47. H. Gu, B. Hu, J. Li, S. Yang, J. Han, H. Chen*, “Rapid Analysis of Aerosol Drugs Using Nano Extractive Electrospray Ionization Tandem Mass Spectrometry”, Analyst, 135, 1259-1267, 2010. PMID: 20498877
  48. B. Hu, X. Peng, S. Yang, H. Gu, H. Chen*, Y. Huan, T. Zhang, X. Qiao, “Fast Quantitative Detection of Cocaine in Beverages Using Nanoextractive Electrospray Ionization Tandem Mass Spectrometry”, J. Am. Soc. Mass Spectrom., 21, 290-293, 2010. PMID: 19939702
  49. H. Gu, Z. Pan, B. Xi, B. E. Hainline, N. Shanaiah, V. Asiago, A. N. Gowda, D. Raftery*, “1H NMR Metabolomics Study of Age Profiling in Children”, NMR Biomed., 22, 826-833, 2009. PMCID: PMC4009993
  50. S. Yang, J. Han, Y. Huan, Y. Cui, X. Zhang, H. Chen, H. Gu*, “Desorption Electrospray Ionization Tandem Mass Spectrometry for Rapid Detection of 24 Carcinogenic Aromatic Amines in Textiles”, Anal. Chem., 81, 6070-6079, 2009.
  51. J. Ding, H. Gu, S. Yang, M. Li, J. Li, H. Chen*, “Selective Detection of Diethylene Glycol in Toothpaste Products Using Neutral Desorption Reactive Extractive Electrospray Ionization Tandem Mass Spectrometry”, Anal. Chem., 81:8632-8638, 2009. PMID: 19761215
  52. S. Yang, H. Chen*, Y. Yang, B. Hu, X. Zhang, Y. Zhou, L. Zhang, H. Gu, “Imaging Melamine in Egg Samples by Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry”, Chin. J. Anal. Chem., 37, 315-318, 2009.
  53. A. N. Gowda, S. Zhang, H. Gu, V. Asiago, N. Shanaiah, D. Raftery*, "Metabolomics-Based Methods for Early Disease Diagnostics: A Review", Expert Rev. Mol. Diagnos., 8, 617-33, 2008. PMCID: PMC3890417
  54. H. Gu, Z. Pan, C. Duda, D. Mann, C. Kissinger, C. Rohde, D. Raftery*, “1H NMR Study of the Effects of Sample Contamination in the Metabolomics Analysis of Mouse Urine”, J. Pharm. Biomed. Anal., 45, 134-140, 2007. PMID: 17707608
  55. H. Gu, H. Chen, Z. Pan, A. U. Jackson, N. Talaty, B. Xi, C. Kissinger, C. Duda, D. Mann, D. Raftery*, R. G. Cooks*, “Monitoring Diet Effects from Biofluids and Their Implications for Metabolomics Studies”, Anal. Chem., 79, 89-97, 2007. PMID: 17194125
  56. H. Chen, Y. Sun, A. Wortmann, H. Gu, R. Zenobi*, “Differentiation of Maturity and Quality of Fruit Using Noninvasive Extractive Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry”, Anal. Chem., 79, 1447-1455, 2007. PMID: 17297943
  57. Z. Pan, H. Gu, N. Talaty, H. Chen, B. E. Hainline, R. G. Cooks*, D. Raftery*, “Principal Component Analysis of Urine Metabolites Detected by NMR and DESI-MS in Patients with Inborn Errors of Metabolism”, Anal. Bioanal. Chem., 387, 539-549, 2007. PMID: 16821030